![]() On field sheet, record sample ID and check the "Bacteria" column. It is important to leave the air space so that the sample will constantly mix while transported. Replace the cap, leaving 1" of air space in the bottle. Be sure not to collect any sediment, and avoid collecting any water from the surface layer of the river as this is uncharacteristic of the water flowing through. ![]() If there is no current, create a current artificially by pushing bottle forward horizontally in a direction away from the hand. Point the bottle opening upstream, into the current. Keep the bottle closed until just ready to sample. Take a sample in mid-stream, or if not possible, from as far out from shore as is safe. Wait for pre-disturbance (from wading in) conditions Sample should be taken from representative, flowing water.Ĭarefully wade in to the stream, walking upstream and avoid to stir up bottom sediment. If you are sampling from waters known to be contaminated with sewage, wear latex gloves to protect yourself Wash hands before starting run - if you sample in an area that may have high fecal levels rinse hands before handling the next sample bottleīe careful not to touch your hands to yourself before you have cleaned them in order to avoid coming into contact with pathogens. ![]() Sterile High Density Polyethylene (HDPE) bottle, 250 ml.Department of Environmental Protection-approved SOP, get the pdf file here Equipment geometric mean standard of 35 enterococcus bacteria per 100 ml of water for five samples over 30 days and an instantaneous (single sample) standard of 104 enterococcus bacteria per 100 ml of water (salt water).geometric mean of 33 enterococcus bacteria or 126 E.coli bacteria per 100 ml of water (fresh water).1000 colonies of fecal coliform bacteria /100ml for secondary contact recreation. ![]() 200 colonies of fecal coliform bacteria /100ml for primary contact recreation.River Bacteria State Standards for bacteria Recreational use: ![]()
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